A thermocline (also known as the thermal layer or the metalimnion in lakes) is a distinct layer based on temperature within a large body of fluid (e.g. water, as in an ocean or lake; or air, e.g. an atmosphere) with a high gradient of distinct temperature differences associated with depth. In the ocean, the thermocline divides the upper mixed layer from the calm deep water below.

Depending largely on season, latitude, and turbulent mixing by wind, thermoclines may be a semi-permanent feature of the body of water in which they occur, or they may form temporarily in response to phenomena such as the radiative heating/cooling of surface water during the day/night. Factors that affect the depth and thickness of a thermocline include seasonal weather variations, latitude, and local environmental conditions, such as tides and currents.